PReS-FINAL-2100: Methotrexate treatment affects effector, but not regulatory T cells in juvenile idiopathic arthritis
نویسندگان
چکیده
Methods Peripheral blood samples were obtained from JIA patients at MTX start and 3 and 6 months thereafter. Treg numbers and phenotype were determined by flow cytometry and suppressive function in allogeneic suppression assays. Teff proliferation upon stimulation with anti-CD3, activation status and intracellular cytokine production were determined by flow cytometry. Effector cell responsiveness to suppression was investigated in autologous suppression assays. Effector cell cytokines in supernatants of proliferation and suppression assays and in plasma were measured by cytokine multiplex assay.
منابع مشابه
PReS-FINAL-1003: Intrinsic cd4 and cd8 effector t cell resistance to suppression in the synovial fluid of juvenile idiopathic arthritis patients
Introduction Autoimmune diseases are characterized by an imbalance between regulatory T cells (Treg) and effector T cells (Teff) both in terms of number and function. Our group previously showed that Teff from the site of inflammation (i.e. synovial fluid, SF) of patients affected by Juvenile Idiopathic Arthritis (JIA) are resistant to autologous Treg-mediated suppression irrespective of the so...
متن کاملMethotrexate treatment affects effector but not regulatory T cells in juvenile idiopathic arthritis.
OBJECTIVE The balance between Treg and effector T cells (Teff) is crucial for immune regulation in JIA. How MTX, the cornerstone treatment in JIA, influences this balance in vivo is poorly elucidated. The aim of this study was to investigate quantitative and qualitative effects of MTX on Treg and Teff in JIA patients during MTX treatment. METHODS Peripheral blood samples were obtained from JI...
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Introduction Juvenile idiopathic arthritis (JIA) is the most common arthritis disease of childhood and is an important cause of disability. Methotrexate (MTX) is the mainstay treatment in JIA. Unfortunately, 30-35% of patients fail to respond MTX, and the delay in identifying the optimal treatment at an early stage of disease can lead to longterm joint damage. Recent studies have evaluated comp...
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عنوان ژورنال:
دوره 11 شماره
صفحات -
تاریخ انتشار 2013